What is M13 phage?
M13 PHAGE Bacteriophage M13 was first isolated from wastewater in Munich (Hofschneider, 1963). Hence named as M13 phage. It is a filamentous phage which has 6407 nucleotides. It possess single stranded circular DNA. It was sequenced by Sanger in 1982. 6.
What is λ phage?
1. BACTERIOPHAGE CLONING VECTORS (λ AND M13) Lecture-4 1 3. INRODUCTION •Esther Lederberg found E.coli K12 to be lysogenic for λ phage, which marked the discovery of λ phage as well as phenomenon of lysogeny •This is a temprate phage •It is a lamboid phage that can infect E.coli 3
What are substitution vectors in phage?
Phage that have a stuffer fragment are called substitution vectors because they are designed to have a piece removed and substituted with something else. Examples are Lambda phage, M13 phage, T4,T7 phage, P1 phage etc.
What are the bacterial hosts for M13 vector?
31. Bacterial hosts for M13 vector •Only male (F+) bacteria are used for proliferation of M13 phage because it enters the host cell through sex pilli encoded by F factor •Female bacteria can be infected by transfection 31
